Xpress epitope tag. 0 Da 2 segments Segment 2: enterokinase site 588 .

Xpress epitope tag. However, if the His6 tag were to be absent, then the Xpress tag would also be non-existent. This sequence includes an ATG translation initiation codon, a polyhistidine tag that functions as a metal binding domain in the translated protein, a transcript stabilizing sequence from gene 10 of phage T7, the XpressTM epitope, and the enterokinase cleavage recognition sequence. Xpress Epitope Antibody, supplied by Thermo Fisher, used in various techniques. Jan 10, 2023 · X-press Tag Peptide Biological Activity： X-press Tag Peptide is a tag peptide used for protein purification. Designed for its small size and high specificity, this epitope tag enables researchers to purify, detect, and study proteins in complex biological systems with unparalleled precision. Proteins that are tagged with His6 can be easily purified through affinity chromatography using nickel or cobalt-based Epitope tags include V5-tag, c-myc-tag, and HA-tag. The T7 epitope allows for efficient and specific detection, purification, and localization of the tagged protein. 5 Da Product: Xpress™ epitope tag, including an enterokinase recognition and The fusion gene was flanked by a histidine tag, Xpress epitope and enterokinase (EK) cleavage site on its 5 -end. 2 segments Segment 2: enterokinase site 178 . 4 Da Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site Xpress™ tag 579 . The N-terminal peptide (XpressTM epitope and 6xHis tag) will add ~ 3 kDa to the size of your protein. 2nd: binds to primary antibodies, amplification for the signal provided by the primary antibody. Why did we use a primary antibody that recognizes the Xpress epitope tag and not the His 6 tag to detect rGFP? Epitope tag antibodies are very commonly used to study localization of a target protein in immunofluorescence (IF) assays. This N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of the bacteriophage T7 gene 10 protein), and an enterokinase cleavage site. Description: X-press Tag is an N-terminal leader peptide, this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10 protein) and an enterokinase cleavage site. Background/Target Information Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. 490 = 9 bp 3 amino acids = 409. 5 Da Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site We use the Xpress epitope tag because we need to detect the antibody that bound to the There is a light band at the W2 position. Because the fraction that exhibits the most fluorescence is assume to be the one with the most rGFP, any degradation is unlikely to occur in the His6 -Xpress epitope region. The reason we use antibodies that recognize the Xpress epitope tag and not the His 6 tag is because in theory every that eluted out will have the His6 tag. All pcDNA™ vectors contain a strong promoter for high-level expression in mammalian cells, a choice of selection marker for generating stable cell lines, and an epitope tag for easy detection with a monoclonal antibody and rapid purification on nickel-chelating resin. The Flag-tag is an artificial tag and, based on the peptide sequence, is also called DYKDDDDK-tag. tag für ‚Markierung‘, ‚Schildchen‘ oder ‚Etikett‘) werden in der Biochemie verschiedene, meist kurze Aminosäuresequenzen bezeichnet, mit deren Hilfe Proteine markiert werden können. 5749 = 24 bp 8 amino acids = 998. 881 = 19 bp promoter for bacteriophage T7 RNA polymerase T7 promoter 863 . 881 Nov 19, 2012 · TMV-Gate vectors that allow addition of N-terminal epitope tags, such as HA, 3xHA, FLAG, 3xFLAG, c-myc, 3xc-myc and 2xStrep-tag II-6xHis-Xpress epitope tag are designated as pSK101-pSK106 and Nov 1, 2024 · X-press Tag is also an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10 protein) and an enterokinase cleavage site. ZERO BIAS - scores, article reviews, protocol conditions and Epitope tags are an extremely useful tool for all types of research. 5 Da Product: Xpress™ epitope tag, including an enterokinase recognition and Introduction The Anti-Xpress and Anti-Xpress -HRP (horseradish peroxidase-conjugated) Antibody allows detection of recombinant proteins containing the N-terminal leader peptide (Xpress epitope). The main function of the T7 epitope is to facilitate the Sep 27, 2019 · Epitope tags are widely used in various applications, but often lack versatility. Using the antibody to recognize the tag would also lower the number of contaminants that would be tagged. Product Data Sheet X-press Tag Peptide -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen) Epitope tags include ALFA-tag, V5-tag, Myc-tag, HA-tag, Spot-tag, T7-tag and NE-tag. Apr 24, 2005 · Yeast that only expressed the Xpress epitope tag linked to Aga2 were prepared in parallel by transfecting EBY100 cells with the parent vector pYD1. 5726 = 24 bp 8 amino acids = 998. The tags are relatively small polypeptides, ranging from a few to several tens of amino acids in length. However, there are many things to consider before using epitope tags to ensure that experiments go as planned. 4 Da Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site Xpress™ tag 482 . 0 Da 2 segments Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site T7 terminator 302 169 . They enable the detection of the protein in the cell and allow the purification of the associated protein complex. • N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody • N-terminal Xpress™ epitope for detection with the Anti-Xpress™ Antibody • Enterokinase cleavage site for removal of fusion tag A set of three vectors is provided (A, B, and C). MedChemExpress X-press Tag Peptide is a tag peptide used for protein purification. 5 Da Product: Xpress™ epitope tag, including an enterokinase recognition and Aug 20, 2015 · On the other hand, “epitope-tag purification, such as with HA, C-Tag, Myc and FLAG, require more expensive media due to the fact that each of these require an immobilized antibody directed against the short epitope-tag sequence,” he says. 载体标签： 6x His Tag（N-端）：用于镍亲和层析纯化和Anti-HisG抗体检测。 Xpress Epitope Tag（N-端）：用于Anti-Xpress抗体检测和分析。 Enterokinase (EK) 切割位点：用于在纯化后去除N-端标签。载体抗性：氨苄青霉素（Ampicillin）。克隆菌株：TOP10。表达菌株：推荐使用LMG194。稳定性：稳表达。组成型/诱导型 X-press标签多肽 (X-press Tag Peptide)是一种N-端前导肽，Anti-Xpress抗体可以识别Xpress表位，因此，X-press Tag多肽可用来纯化X-press Tag融合蛋白。 Dec 11, 2014 · Protein tags can be used for purification, detection, localization, and more. 3 amino acids = 409. The primary antibody that recognizes the xpress epitope tag was used instead of the one for the His6 tag to detect rgfp because the His6-tag is not very immunogenic, making it less suitable for detection in X-press Tag Peptide is a tag peptide used for protein purification. 编号: 188560 中文名称: 多肽标签X-press 3 amino acids = 409. Antibodies that recognize a tag are commonly called tag antibodies, and the tag sequence that serves as an epitope for tag antibodies is called an “epitope tag”. Flag-tag is used to tag proteins for multiple capture and detection applications. Jun 10, 2014 · There is a wide variety of protein tags that can be used to suit your purpose. Both vectors offer the URA3 gene for selection in yeast. 5749 = 15 bp 5 amino acids = 606. Description of X-press Tag Peptide X-press Tag is an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10?protein) and an enterokinase cleavage site. Its flexibility and compatibility with various detection methods make it a cornerstone of modern molecular Xpress Epitope Tagwere, supplied by Thermo Fisher, used in various techniques. Epitope Tags in Protein Research Recombinant DNA technology allows researchers to construct fusion proteins which express the gene of interest fused with an epitope tag. We would like to show you a description here but the site won’t allow us. 0 Da 2 segments Segment 2: enterokinase site 5735 . 5726 = 15 bp 5 amino acids = 606. ZERO BIAS - scores, article reviews, protocol conditions and more X-press Tag Peptide - X-press Tag Peptide is a tag peptide used for protein purification. These include affinity tags, epitope tags and fluorescent tags. These tags are particularly useful for western blotting, immunofluorescence and immunoprecipitation experiments, although they also find use in antibody purification. This tag is utilized extensively in molecular biology for the purification of proteins that are overexpressed in various organisms such as bacteria, yeast, or mammalian cells. pRSET A vector Map Copy Sequence View Map pRSET A 2897 bp 600 1200 1800 2400 T7 promoter RBS ATG 6xHis T7 tag (gene 10 leader) Xpress (TM) tag MCS T7 terminator f1 ori AmpR promoter AmpR ori Xpress Epitope Tag, supplied by Thermo Fisher, used in various techniques. 505 = 24 bp 8 amino acids = 998. Known genes fused to epitope tags can be easily detected or purified using well-characterized tag antibodies. 579 . pYES2/NT features an N-terminal Xpress™ epitope for detection with Invitrogen's Anti-Xpress™ Antibody and a polyhistidine (6xHis) tag for purification with nickel-chelating resin. X-press Tag is also an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10 protein) and an enterokinase cleavage site. 0 Da 2 segments Segment 2: enterokinase site 178 . Bioz Stars score: 86/100, based on 1 PubMed citations. Learn more about the molecular tool box of tags and fusion proteins. A Western blotting experiment using Anti Xpress Antibody (Invitrogen) raised against the Tag polypep - tide was performed to confirm that the epitope had been cleaved from the fused CAT protein. The Anti-Xpress antibodies recognize the 169 . 4 Da Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site Xpress™ tag 169 . X-press Tag Peptide is a tag peptide used for protein purification. Xpress Epitope Tag, supplied by Thermo Fisher, used in various techniques. Jun 6, 2016 · Epitope tags are widely used in cell biology and biochemical analysis 1. heep anti-mouse IgG polyclonal anti-serum conjugated horse radish peroxidase. The primary antibody is used to recognize the Xpress epitope tag and not the His6 tag, due to the Xpress epitope tag being specific to rGFP. cerevisiae expression vectors derived from the parental pYES2 vector. Epitope tags provide a method to localize gene products in a variety of cell types, study the topology of proteins and protein complexes, identify associated proteins, and characterize newly identified, low abundance or poorly immunogenic proteins when protein specific antibodies are not available. c Common site-specific proteases are described in Table 4. Each vector is available in three reading frames to simplify cloning in-frame with the fusion tag. Raising polyclonal antibodies is time-consuming and expensive; raising monoclonal antibodies even more so. Many Xpress Epitope, supplied by Thermo Fisher, used in various techniques. Epitope tag detection with an unconjugated antibody requires the 579 . Bases on the visibility of the band, we could conclude that the protein in this rGFP (279 aa) - 23 (N-terminus degradation along with His 6 tag aa) = protein BIOl 3380 - Lab 7 Report Course: Biochemistry Laboratory Aug 4, 2022 · The xpress epitope tag was used over the His6 tag to detect rgfp due to its higher immunogenicity and the practicality of using a single antibody to detect multiple epitope-tagged proteins. (5pts) Make a figure of your Western blot result with appropriate titles and labels. It is derived from the T7 bacteriophage gene 10. Why do we use antibodies that recognize the Xpress epitope tag and not the His6 tag? west blot There are approximately 20 other proteins in E. 6 days ago · X-press Tag is also an N- terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10 protein) and an enterokinase cleavage site. we use primary antibodies that recognize the Xpress epitope tag and not the his6 tag to detect rGFP because using antibodies that can detect his-tag will get bind to it. 0 Da 2 segments Segment 2: enterokinase site 588 . Feb 29, 2024 · The His6 Xpress epitope tag consists of six histidine amino acids in a row, hence the name 'His6'. 0 Da 2 segments Segment 2: enterokinase site 5712 . X-press Tag Peptide 是一种N-端前导肽，Anti-Xpress 抗体能够识别 Xpress 表位，因此，该多肽可用来纯化 X-press Tag 融合蛋白。- 高纯度，全球文献引用。 Description X-press Tag Peptide is a tag peptide used for protein purification. pYES2/CT contains a C-terminal V5 epitope for detection May 22, 2025 · ChemicalBook 为您提供Xpress Tag Peptide的化学性质，熔点，沸点，密度，分子式，分子量，物理性质，毒性，结构式，海关编码等信息，同时您还可以浏览Xpress Tag Peptide产品的价格，供应商，贸易商，生产企业和生产厂家，最后Xpress Tag Peptide的中文，英文，用途 CAS cas number cas no可能也是您需要的。 Nov 8, 2020 · The primary antibody is used to recognize the Xpress epitope tag and not the His 6 tag, due to Xpress epitope tag being specific to rGFP. 505 = 15 bp 5 amino acids = 606. pYES2/NT and pYES2/CT are S. Epitope tag detection with an unconjugated antibody requires the use of a secondary antibody X-press Tag Peptide is a tag peptide used for protein purification. Thermo Fisher untuk analisis western blot menggunakan antibodi monoklonal xpress epitope yang mengandung histidine tag Untuk Analisis Western Blot Menggunakan Antibodi Monoklonal Xpress Epitope Yang Mengandung Histidine Tag, supplied by Thermo Fisher, used in various techniques. Background X-press Tag is an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10?protein) and an enterokinase cleavage site. Begrifflich und funktionell analog, aber auf Nukleinsäure-Ebene, werden Thermo Fishern terminal xpress epitope tagN Terminal Xpress Epitope Tag, supplied by Thermo Fisher, used in various techniques. Nov 13, 2024 · The Flag-tag peptide (DYKDDDDK) is an invaluable molecular tool in biotechnology and biomedical research. X-press Tag is also an N-terminal leader peptide; th Targetmol: X-press Tag: an N-terminal peptide with polyhistidine, T7 gene 10 Xpress epitope, and enterokinase site, detected by anti-Xpress antibodies. 0 Da 2 segments Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site T7 promoter 863 . 602 = 24 bp 8 amino acids = 998. X-press Tag Peptide is a tag peptide used for protein purification. 5 Da Product: Xpress™ epitope tag, including an enterokinase recognition and Unique among epitope tags, GFP is an autofluorescent 27 kDa tag that can be directly detected in living cells by fluorescent microscopy. Oct 29, 2023 · The Xpress epitope tag is specific to the protein of interest: rGFP, and as such antibodies binding to this tag would indicate, exclusively, the presence of rGFP. . Epitope tags (also known as fusion tags or afinity tags) ofer a convenient solution to this problem by acting as universal epitopes for detection and purification without disturbing the structure of the protein to which they are fused. Epitope tags are powerful tools for protein characterization. 0 Da 2 segments Segment 2: enterokinase site 491 . ZERO BIAS - scores, article reviews, protocol conditions and more X-press Tag is an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10u2028protein) and an enterokinase cleavage site. Anti-FLAG M2 is the most popular antibody agai…. Among them, the FLAG® (DYKDDDDK, DDDDK, etc N-terminal polyhistidine (6xHis) tag for rapid purification with nickel-chelating resin and detection with an Anti-HisG Antibody N-terminal Xpress epitope for detection with the Anti-Xpress Antibody 482 . Hemagglutinin A (HA) Derived from the binding domain of the Influenza hemagglutinin protein, HA contains a high proportion of charged residues (YPYDVPDYA) so it is likely to form a strong antibody recognition X-press Tag Peptide is a tag peptide used for protein purification. However only the rGFP will have the Xpress epitope tag allowing us to weed out contaminants. Aug 15, 2024 · The FLAG-tag/anti-FLAG system is a widely used biochemical tool for protein detection and purification. 4 Da Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site Xpress™ tag 5703 . 1012 = 24 bp 8 amino acids = 998. 177 = 9 bp 3 amino acids = 409. Your immune system creates defensive proteins called antibodies. X-press Tag is also an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence， the Xpress epitope (part of bacteriophage T7 gene 10 protein) and an enterokinase cleavage site. Here, the authors introduce a small, alpha helical tag, which is recognized by a high affinity nanobody and can be Mar 9, 2015 · N-terminal fusion peptide encoding: 6xHis tag for protein purification using metal binding resins Xpress epitope for detection of the expressed fusion protein using an Anti-Xpress antibody Enterokinase (EK) recognition site for efficient cleavage of the fusion peptide from the FP Fluorescent Protein derived from eGFP (CFP, EmGFP, BFP) Feb 19, 2016 · An epitope is a short sequence of amino acids on an antigen, which an antibody attaches itself to. If we were to use antibodies that recognize the His6 tag it would bind to other proteins, not just rGFP. X-press Tag is an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10 protein) and an enterokinase cleavage site. Die derart markierten Proteine gehören zu den Fusionsproteinen. Unconjugated antibodies let you choose the detection method which best suits your needs. This will create a fusion with the N-terminal polyhistidine tag, XpressTM epitope, and the enterokinase cleavage site. The HSV, T7-tag, Tag100, V5, Xpress, and c-myc fusions provide epitopes for well-characterized, commercially available antibodies. ZERO BIAS - scores, article reviews, protocol conditions and more This sequence includes an ATG translation initiation codon, a polyhistidine tag that functions as a metal binding domain in the translated protein, a transcript stabilizing sequence from gene 10 of phage T7, the Xpress epitope, and the enterokinase cleavage recognition sequence. primary antibody we used is a mouse IgG anti-Xpress epitope monoclonal antibody that was raised against the "Xpress" epitope that is present in the expression vector sequence. 5 Da Product: Xpress™ epitope tag, including an enterokinase recognition and Thereafter, the epitope-tagged fusion protein can be either detected or purified using an antibody specific for the epitope tag. The BamHI and HindIII restriction sites were located at its 5 -and 3 -end Epitope-tagged proteins can be purified using immobilized primary antibodies to the epitope tag although antibody affinity chromatography often involves a low or high pH elution which can irreversibly affect the properties of the fusion protein, and employs resin which has limited reusability. 602 = 15 bp 5 amino acids = 606. Flag-tag is a commonly used epitope or peptide tag that is not derived from a natural protein. coli that behave like rGFP and contain histidines. 5. Anti-Xpress antibodies recognize the Xpress epitope sequence found in this leader peptide. May 18, 2015 · For example Life Technologies has several different constructs with His-Tag on N or C-Terminus and Xpress Epitope tag. Without the presence of either The Anti-Xpress (46-0528) antibody is a monoclonal antibody that specifically recognizes the Xpress epitope tag, which is commonly used for the expression and purification of recombinant proteins. An epitope tag can either be fused at C- or N-terminus of a target gene and expressed in a desired expression system. Epitope tagging is an efficient way to study the following scenarios: Proteins whose antibodies are not Nov 17, 2022 · X-press Tag is also an N-terminal leader peptide; this N-terminal peptide contains a polyhistidine sequence, the Xpress epitope (part of bacteriophage T7 gene 10 protein) and an enterokinase cleavage site. It contains the XpressTM epitope for simple detection with Invitrogen’s Anti-XpressTM Antibody and a polyhistidine (6xHis) tag for purifi-cation using nickel resin. 5 Da Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site X-press Tag是N端先导肽;这个n端肽含有一个多组氨酸序列、一个Xpress表位 (噬菌体T7基因10蛋白的一部分)和一个肠激酶裂解位点。 The pelB and gene III signal peptides direct periplasmic localization of fusion proteins. Posttranslational modifications also cause the protein to migrate differently than expected. Many different types of epitope tags have been developed for different purposes. 192 = 24 bp 8 amino acids = 998. 4 Da Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site Xpress™ tag 5726 . Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site Xpress™ tag 989 . This antibody can be used in various applications, including Western blotting, immunoprecipitation, and immunofluorescence, to detect and monitor the expression of Xpress-tagged proteins. Question: Why did we use a primary antibody that recognizes the Xpress epitope tag and not the His6 tag to detect rGFP? Why did we use a primary antibody that recognizes the Xpress epitope tag and not the His 6 tag to detect rGFP? There are 2 steps to solve this one. 5 Da Product: Xpress™ epitope tag, including an enterokinase recognition and cleavage site Xpress™ tag 169 . 587 = 9 bp 3 amino acids = 409. Epitope tag detection with an unconjugated antibody requires the use of a secondary antibody that carries a reporter for detection. X-press Tag Peptide 是一种N-端前导肽，Anti-Xpress 抗体能够识别 Xpress 表位，因此，该多肽可用来纯化 X-press Tag 融合蛋白。 X-press-Tag是一种N-末端前导肽；该N末端肽包含一个多组氨酸序列，即Xpress表位（噬菌体 T7基因10的一部分蛋白质）和肠激酶切割位点。 primary antibody we used is a mouse IgG anti-Xpress epitope monoclonal antibody that was raised against the "Xpress" epitope that is present in the expression vector sequence. T7 epitope tag for protein purification The T7 epitope is a short amino acid sequence (MASMTGGQQMG) that is commonly used as a tag in protein expression and purification. The vector is supplied in three reading frames to facilitate cloning. Fluorescence tags are used to give visual readout on a protein. This article goes through various topics, such as how to select an epitope tag, design an epitope tag fusion construct, improve sensitivity, how to combine tags, and a troubleshooting guide for when no/low expression Explore widely-used epitope tags, such as c-myc and HA, including recommended applications and what to watch out for. ZERO BIAS - scores, article reviews, protocol conditions and more Als Protein-Tag, Affinitäts -Tag oder Epitop -Tag (engl. ZERO BIAS - scores, article reviews, protocol conditions and more Epitope Tag Antibodies Simplify your protein research with GenScript’s high-quality epitope tag antibodies. 192 = 15 bp 5 amino acids = 606. snt1qmre yl mhrkj gd 5avr3 pyagmy 15y ykyu6xmie u6vi76 ryl