10x chromium next gem protocol 1 is a high throughput solution on the Chromium X series instrument for analyzing hundreds of thousands of cells per run, with up to Chromium Next GEM Single Cell 5ʹ Reagent Kits v2 (Dual Index) 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Recommended Videos How it works: Universal 5' Gene Expression Chromium GEM-X intro webinar: See the next generation of single cell in action Data analysis For guidance on qualifying the Chromium Controller or Chromium Single Cell Controller, refer to the Chromium Controller Specifications (CG00020) or the Chromium Single Cell Controller Get started with your Chromium Single Cell Immune Profiling experiment. Versatile assays are Following single-cell dissociation or nuclei isolation, the Next GEM 3' assay uses microfluidics to partition and assign cell- or nuclei-specific barcodes to transcript cDNA at the 3' end. FOR USE WITH Chromium Controller & Next GEM Accessory Kit, PN-1000202 (Includes Chromium Next GEM Test Chip, PN-2000199) Nuclei is isolated from frozen post-mortem CNS samples. 10x The final library products include the single nucleus whole transcriptome library, which contains the gene expression information, and the Trekker library, which contains the spatial location of Chromium Next GEM Single Cell 3’ Reagent Kits v3. This series of videos will walk you through the Chromium Next GEM Chromium Next GEM Training Reagents, Gel Beads and Chip Kits Following single-cell dissociation or nuclei isolation, the Next GEM 3' assay uses microfluidics to partition and assign cell- or nuclei-specific barcodes to transcript cDN This protocol has been adapted from the 10X User Guide CG000420, Rev D. In this initial The 10x Genomics Multiome assay enables simultaneous profiling of gene expression and open chromatin within the same cell across thousands of cells (ref 1-2), offering a compreh Stepwise Objectives The Chromium Single Cell Gene Expression Solution with Feature Barcoding technology upgrades short read sequencers to deliver a scalable microfluidic platform for This series of videos will walk you through the Chromium Next GEM ATAC v2 protocol. The technique makes use of the Chromium system, Get started with your Chromium Single Cell Immune Profiling experiment. Stepwise Objectives The Chromium Single Cell Gene Expression Solution with Feature Abstract Following single-cell dissociation or nuclei isolation, the Next GEM 3' For use with: Chromium GEM-X Single Cell 3' Kit v4, 16 rxns PN-1000691 | 4 rxns PN-1000686 Chromium GEM-X Single Cell 3' Chip Stepwise Objectives The Chromium Single Cell Gene Expression Solution upgrades short read sequencers to deliver a scalable microfluidic platform for 3ʹ digital gene expression by profiling Introduction Chromium Next GEM Single Cell 3' HT v3. RNA/ATAC sequencing is performed according to the 10X Genomics’ Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & For use with: Chromium GEM-X Single Cell 3' Kit v4, 16 rxns PN-1000691 | 4 rxns PN-1000686 Chromium GEM-X Single Cell 3' Chip Introduction Chromium Next GEM Single Cell 3' HT v3. This video Single cell detection of intracellular oncogenic protein markers with multiplexed GEM-X Flex Scaling comparative lung cancer studies using Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 4 rxns PN-1000285, includes: Chromium Next GEM Single Cell Multiome ATAC Kit A, 4 rxns PN-1000281 Chromium Next GEM Single Cell ATAC Reagent Kits v1. 1 | Assemble Chromium Next GEM Chip G Once you’ve prepared the master mix, you are ready to assemble Chromium Next GEM Chip G. Single cell CRISPR screens Refer to the Chromium Next GEM Single Cell ATAC Reagent Kits v2 User Guide for the complete protocol. com The Chromium Next GEM Single Cell 5’ HT v2 assay enables partitioning of 2,000–20,000 cells per channel of the Chromium Next GEM Chip N. . In this initial video, find out more about the Chromium Next GEM Single Cell ATAC kits, including Once you’ve prepared the master mix, you are ready to assemble Chromium Next GEM Chip K. However, the structure and clarity of the 10X Genomics User Introduction Chromium Next GEM Single Cell 5' HT v2 is a high throughput solution on the Chromium X series instrument for analyzing hundreds of thousands of cells per run, with up to FOR USE WITH: Chromium Next GEM Single Cell 3’ GEM, Library & Gel Bead Kit v3. 1 (Dual Index) 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & This series of videos will walk you through the Chromium Next GEM Single Cell Gene Expression Flex protocol. Powered by Next GEM 10xgenomics. All 10x Genomics single cell dual index Chromium Next GEM Single Cell V(D)J Reagent Kits v1. Up to 16 samples loaded Chromium Next GEM Single Cell 3’ Reagent Kits 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Introduction The Chromium Single Cell Immune Profiling Solution provides a comprehensive, scalable solution to simultaneously examine the cellular context of the immune system and the Chromium Next GEM Single Cell ATAC Reagent Kits v2 Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Recommended Pipette Tips Protocol Once you’ve prepared the master mix, you are ready to assemble Chromium Next GEM Chip L. This video provides step-by-step instructions for loading the Chromium Next GEM Chip G, including Chromium Next GEM Single Cell Multiome ATAC + Gene Expression Reagent Bundle, 16 rxns PN-1000283, includes: Chromium Following single-cell dissociation or nuclei isolation, the Next GEM 5' assay uses microfluidics to partition and assign cell- or nuclei The 10x Chromium Next GEM technology partitions cells or nuclei into droplets containing uniquely barcoded beads called GEMs (Gel Bead-In Emulsions). 1 (Dual Index) 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & FOR USE WITH: CG000169: Nuclei Isolation for Single Cell ATAC Sequencing CG000212: Nuclei Isolation from Mouse Brain Tissue Following single-cell dissociation or nuclei isolation, the Next GEM 3' assay uses microfluidics to partition and assign cell- or nuclei-specific barcodes to transcriptcDNA at the 3' Automating the 10X Genomics Chromium Next GEM Single Cell Multiome ATAC + Gene Expression workflow offers significant benefits, including increased throughput, Load Chip G immediately after combining the master mix, water and single cell suspension. 1), a Master Mix containing CellPlex labeled cells, and Partitioning Oil onto Next GEM Chromium The Chromium Single Cell platform was designed to help make single cell studies accessible and approachable for everyone. 1, 16 rxns PN-1000121 Chromium Next GEM For guidance on qualifying the Chromium Controller or Chromium Single Cell Controller, refer to the Chromium Controller Specifications (CG00020) or the Chromium Single Cell Controller The Chromium platform, powered by Next GEM technology, enables integrated analysis of single cells at massive scale. 1Protocol We do have our own protocol for the single-cell gene expression assay. In combination with multiomic approaches Introduction The Chromium X Series offers a highly optimized approach to single cell studies and enables both low and high throughput experiments. 1 is a high throughput solution on the Chromium X series instrument for analyzing hundreds of thousands of cells per run, with up to Following single-cell dissociation or nuclei isolation, the Next GEM 5' assay uses microfluidics to partition and assign cell- or nuclei-specific barcodes to transcript cDNA at the Load Chip G immediately after combining the master mix, water and single cell suspension. In this initial Load Chip K immediately after combining the master mix, water and single cell suspension. Our suite of Chromium Single Cell products can capture molecular 10x Genomics is a company behind the microfluidics-based method of single-cell RNA sequencing. This series of videos will walk you through the Chromium Next GEM Single Cell 5' v2 Dual Index protocol. 1 Dual Index protocol. 1, 4 rxns PN-1000269 3’ Feature Barcode Kit, 16 rxns PN-1000262 Chromium Next GEM Chip G Single Cell Kit, 48 rxns PN-1000120 Different single-cell assays require different chips, which have names such as the Next GEM Chip G or Next GEM Chip Q. This video provides a look at best practices for HT chip assembly, including tips for HT chip Chromium Next GEM Single Cell 3' Reagent Kits 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Introduction Chromium Next GEM technology enables profiling the whole transcriptome of hundreds to tens of thousands of cells per sample. This series of videos will walk you through the Chromium Next GEM Single Cell 3' v3. 10X Genomics Universal 3’ /5’ Gene Expression (GEM-X or Next-GEM) Profiling of gene expression levels at single-cell resolution. 1 Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Chromium Next GEM Single Cell 3ʹ Reagent Kits Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Objectives Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Chromium Next GEM Single Cell 3ʹ Kit v3. Chromium Next GEM Single-Cell 3’ Kit v3. A pool of Get started with your Chromium Single Cell Gene Expression experiment. This video provides step-by-step instructions for loading the Chromium Next GEM Chip G, including Cell encapsulation, reverse transcription (RT), cDNA amplification, and library preparation was done with the 10X Genomics Chromium Next GEM Single Cell 3ʹ GEM Kit For guidance on qualifying the Chromium Controller or Chromium Single Cell Controller, refer to the Chromium Controller Specifications (CG00020) or the Chromium Single Cell Controller Chromium Next GEM Single Cell 3’ Reagent 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Objectives A recent independent study comparing 9 scRNA-seq technologies found that, “overall, 10x FRP [Next GEM Flex] exhibited the highest ranked analytical Single Cell Gene Expression Protocol v3. This video provides a look at best practices for chip assembly, incl Consult Chromium GEM-X Single Cell Gene Expression v3 & Immune Profiling v4 - Protocol Planner (CG000748) for a detailed list of plastics and other consumables. The Chromium Controller uses advanced microfluidics to perform single cell partitioning and barcoding in a matter of minutes. 1 Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise B. GEM For guidance on qualifying the Chromium Controller or Chromium Single Cell Controller, refer to the Chromium Controller Specifications (CG00020) or the Chromium Single Cell Controller Chromium Next GEM Single Cell 3’ LT Reagent Kits v3. In this initial video, find out more about the Chromium Next GEM Single Cell Chromium Next GEM Single Cell 5' HT v2 is a high throughput solution on the Chromium X series instrument for analyzing hundreds of thousands of cells per run, with up to Chromium Next GEM Single Cell 3’ Reagent Kits 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Chromium Next GEM Single Cell 3’ Reagent Kits 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & For guidance on qualifying the Chromium Controller or Chromium Single Cell Controller, refer to the Chromium Controller Specifications (CG00020) or the Chromium Single Cell Controller Study the complexity of development, disease, gene function, and therapeutic response at single cell resolution. The incorporation of a unique Introduction The Chromium Single Cell Immune Profiling Solution provides a comprehensive, scalable solution to simultaneously examine the cellular context of the immune system and the Get started with your Chromium Single Cell Gene Expression experiment. Created by: Mathew Lyons. This video provides step-by-step instructions for loading the Chromium Next GEM Chip K, including Following single-cell dissociation or nuclei isolation, the Next GEM 5' assay uses microfluidics to partition and assign cell- or nuclei-specific barcodes to transcript cDNA at the Chromium Next GEM Single Cell FLEXProtocol The structure and clarity of the 10X Genomics User Guide is excellent and we highly suggest you simply download and follow along with the FOR USE WITH Chromium Next GEM Single Cell 3ʹGEM, Library & Gel Bead Kit v3. Chromium Next GEM Training Kit User Guide User Guide, CG000210 For guidance on sample preparation for library construction and sequencing, refer to the Following single-cell dissociation or nuclei isolation, the Next GEM 5' assay uses Stepwise Objectives The Chromium Single Cell Gene Expression Solution upgrades short read Chromium Next GEM Single Cell 5' Reagent Kits v2 (Dual Index) 10x Genomics Accessories This protocol has been adapted from the 10X User Guide CG000420, Rev D. 1, 16 rxns PN-1000121 Chromium Next GEM Single Cell 3ʹGEM, Library & Gel Bead Kit v3. In this Workflow Overview GEMs are generated by combining barcoded Single Cell 3' Gel Beads (v3. 1, 4 rxns PN Chromium GEM-X: See the next generation of single cell in action Unlocking cellular secrets: The powerful role of single cell analysis in cell therapy Page topic: "Chromium Next GEM Training Kit - USER GUIDE CG000210 Rev B". Chromium Next GEM Chip K GEMs 10x Barcoded Oil Gel Beads Cells Enzyme Chromium Next GEM Chip K assembled in a Chromium Next GEM Secondary Holder (A). Barcoded The Chromium Single Cell ATAC Solution provides a comprehensive, scalable approach to determine the regulatory landscape Chromium Next GEM Single Cell 5ʹ Reagent Kits v2 (Dual Index) Chromium Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Chromium Next GEM Single Cell 3’ Reagent Kits 10x Genomics Accessories Recommended Thermal Cyclers Additional Kits, Reagents & Equipment Protocol Steps & Timing Stepwise Stepwise Objectives Chromium Single Cell Multiome + Gene Expression provides a comprehensive, scalable multiomic approach for simultaneously profiling epigenomic Stepwise Objectives Chromium Single Cell Multiome + Gene Expression provides a comprehensive, scalable multiomic approach for simultaneously profiling epigenomic Introduction The Chromium Single Cell Gene Expression Solution provides a scalable microfluidic platform for gene expression profiling of 500-10,000 individual cells per sample. 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